Applegate said in an interview with Deadline, “It’s been a great experience working on Up All Night, but the show has taken a different creative direction and I decided it was best for me to move on to other endeavors. Working with Lorne Michaels has been a dream come true and I am grateful he brought me into his TV family. I will miss the cast, producers and crew, and wish them the best always.”
NBC may be looking at former Friends star Lisa Kudrow to replace Applegate on the show, but that has yet to be confirmed. With Up All Night being changed so much here recently and now the departure of Applegate, I think it’s safe to say that it won’t be on the air much longer. This is a shame because it isn’t a bad show - it just hasn’t reached a big enough audience.